The Effect of Alfalfa (Medicago sativa) extract onthe number of macrophage, fibroblast, and amount of collagen in rat wound healing
Hussaana A., Chodidjah, Suwanto Y.A.
Abstract
Background: Alfalfa extract has been shown to have anti-inflammatory activity in wound healing. Objectives: This study aims to investigate the effect of Alfafa on the number of macrophages, fibroblast, and collagen in the rat wound healing. Method: In this post-test-only control group design study, 50 Wistar rats were randomly divided into five groups. K0 (negative control) group was treated with ointment base. K1 (positive control) group was treated with gentamicin. P1, P2, and P3 groups were treated with 10%, 20%, and 30% alfalfa extract respectively. The termination of rats conducted on day 3 and day 7 post of first treatment (5 rats/group/period). On day 3, the number of macrophage and fibroblast were evaluated using Hematoxylin-Eosin staining. On day 7, the number of collagen was evaluated using Sirius-red staining. The data were analyzed using one way ANOVA followed by Tukey’s post hoc test. Result: The result, showed a significant difference in the number of macrophages between the negative control treated group and K1 groups. The number of fibroblasts was significantly different between the P3 group and the P1, K1 group. There was a significant difference in the number of collagens between negative control andtreated group, and between the positive control and P2, P3 group. Conclusion: The administration of alfalfa extract at the dose of 20% can accelerate wound healing by increasing the number of fibroblasts and collagen fibers and decreasing number of macrophages.
The role of intercellular mediators in the regulation of reparative processes of chronic wounds when using photobiomodulation therapy
Babenko N., Kumetchko M., Litvinova O., Pavlov S., Semko N., Valilshchykov M.
Effect of Moringa Leaf Ethanol Extract on Reduced Levels of Mda, TNF-α and Description of Inflammatory Cells in Rat Sinus Mucosa Model of Acute Rhinosinusitis
Indarto D., Purwanto B., Risya Cilmiaty A.R., Wardani A.T.W., Wasita B., Indarto D., Purwanto B., Risya Cilmiaty A.R., Wardani A.T.W., Wasita B.
Insight into the functional and medicinal properties of Medicago sativa (Alfalfa) leaves extract
Karimi E.
Wound-healing promoting effect of total tannins from Entada phaseoloides (L.) Merr. in rats
Diao Y., Li B., Li K., Liu D., Liu X., Pan T., Su X., Wang F., Wang S.
Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action
Galicka A., Jolanta N.
Therapeutic efect of total ginseng saponin on skin wound healing
Bae C.S., Cho I.-H., Cho Y.-S., Go A., Jeong M.-J., Jeong S.-J., Kang S.S., Kim J.C., Kim S.E., Kim S.H., Kim S.H., Kim Y.S., Moon C.J., Nah S.Y.
Macrophage cytokines: Involvement in immunity and infectious diseases
Descoteaux A., Descoteaux A., Duque G.A., Duque G.A., Descoteaux A., Descoteaux A., Duque G.A., Duque G.A.
Anti-inflammatory effects of flavonoids
Cabanillas B., Cabanillas B., Crespo J.F., Maleki S.J., Cabanillas B., Cabanillas B., Crespo J.F., Maleki S.J.
No Title
Chi C. L., Chiriac E. R., Gean E., Sandru C.
In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice
Asrade S., Belachew T.F., Fentahun E., Geta M.
Husein A., Karim M., Liszen T., Alam M.K., Qamruddin I.
Bangladesh Journal of Medical Science
Babenko N., Kumetchko M., Litvinova O., Komarchuk V., Pavlov S.
Zdravotnicke Listy
Sumarawati T., Indriyana W.M., Chodidjah C.
Tropical Journal of Natural Product Research
