Secretome of hypoxia-preconditioned mesenchymal stem cells enhance the expression of HIF-1a and bFGF in a rotator cuff tear model
Ismiarto Y.D., Putra A., Amalina N.D., Alif I., Nazar M.A., Fredianto M., Herman H.
Abstract
Aim To determine the effect of secretome hypoxia mesenchymal stem cells (SH-MSCs) on the relative gene expression of hypoxia inducible factor-1a (HIF-1a) and basic fibroblast growth factor (bFGF) in accelerating histomorphometric repair of tendon to bone interface healing in rats acute rotator cuff tear (RCT) model. Methods This is experimental research with posttest control group design. Thirty-male Wistar rats were divided into five treatment groups: healthy group and rotator cuff reconstruction group included four groups: SH-MSCs W2 (the treatment group was given a SH-MSCs 0.5 mL and terminated at weeks 2), NaCl W2 (the control vehicle group was assigned NaCl 0.5 mL and terminated at weeks 2), SH-MSCs W8 (the treatment group was given a SH-MSCs 0.5 mL and terminated at weeks 8), and NaCl W8 (the control vehicle group was given NaCl 0.5 mL and terminated at weeks 8). All the rats were terminated on day termination and HIF-1a and bFGF gene expression were analysed using qRT-PCR. Results SH-MSCs significantly increased the HIF-1a and bFGF gene expression than the NaCl group even in week 2 and week 8. The highest increased gene expression of HIF-1a and bFGF was on week 8. Conclusion SH-MSCs are important in the healing repair process of the tendon-to-bone interface in acute RCT model rats through increasing gene expression of HIF-1α and bFGF.
Structure Identification of ViceninII Extracted from Dendrobium officinale and the Reversal of TGF-β1-Induced Epithelial–Mesenchymal Transition in Lung Adenocarcinoma Cells through TGF-β/Smad and PI3K/Akt/mTOR Signaling Pathways
Chen H., Du B., Huang S., Huang Y., Lei Z., Li D., Li Y., Luo Y., Ren Z., Wei G., Xing S., Xing S., Chen H., Du B., Huang S., Huang Y., Lei Z., Li D., Li Y., Luo Y., Ren Z., Wei G., Xing S., Xing S., Chen H., Du B., Huang S., Huang Y., Lei Z., Li D., Li Y., Luo Y., Ren Z., Wei G., Xing S., Xing S.
Anti-fibrotic actions of interleukin-10 against hypertrophic scarring by activation of PI3K/AKT and STAT3 signaling pathways in scar-forming fibroblasts
Bai X., Cai W., Fang X., Guan H., Hu D., Hu X., Li J., Shi J., Su L., Wang H., Wang Y., Zheng Z., Zhu X., Bai X., Cai W., Fang X., Guan H., Hu D., Hu X., Li J., Shi J., Su L., Wang H., Wang Y., Zheng Z., Zhu X.
Fibroblasts and myofibroblasts in wound healing
Bonte F., Darby I.A., Desmouliere A., Laverdet B., Bonte F., Darby I.A., Desmouliere A., Laverdet B., Bonte F., Darby I.A., Desmouliere A., Laverdet B.
Collagen I and the fibroblast: High protein expression requires a new paradigm of post-transcriptional, feedback regulation
Schwarz R.I.
Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model
Durselen L., Evans C.H., Heisterbach P., Jaquiery C., Majewski M., Martin I., Muller S.A., Muller S.A., Todorov A.
Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons
Avanessian B., Cao Y., Chen C.H., Liu P.Y., Shimada M., Tang J.B., Tang J.B., Wang X.T., Wang X.T., Wu Y.F., Zhou Y.L.
Conditioned medium derived from FGF-2-modified GMSCs enhances migration and angiogenesis of human umbilical vein endothelial cells
Huang J., Huang J., Huang J., Jin S., Jin S., Jin S., Li S., Li S., Li S., Liu L., Liu L., Liu L., Sun Q., Sun Q., Sun Q., Yang C., Yang P., Yang P., Yang P., Zhang L., Zhang L., Zhang L., Zhang Y., Zhang Y., Zhang Y.
Effect of basic fibroblast growth factor on Achilles tendon healing in rabbit
Abouzar Husseini S, Hussein Lebaschi A, Jaber Mousavi S, Javad Fatemi M, Najafbeygi A, Niazi M.
Wnt/β-catenin signaling enhances hypoxia-induced epithelial-mesenchymal transition in hepatocellular carcinoma via crosstalk with hif-1α signaling
Bai X., Chen C., Chen W., Hu L., Hu Q., Liang C., Liang T., Ma T., Xie S., Xu S., Zhang Q.
The Application of MSCs-Derived Extracellular Vesicles in Bone Disorders: Novel Cell-Free Therapeutic Strategy
Chen Z., Hu L., Liang S., Liu S., Qian A., Xu X., Zhang Y.
Putra A., Setiawan E., Nasution I.S.
Materia Socio Medica
Usman M.A., Suroto H., Johan M.P., Sakti M., Mubarak A.F., Zainuddin A.A., Ruksal Saleh M., Sam A.D.P., Warsinggih W.
Stem Cells and Cloning Advances and Applications
Ho Bhang S., Ho Um S., Hyun J., Won Yun D.
Journal of Industrial and Engineering Chemistry
Altunkaynak B.Z., Piskin A., Kelsaka E., Altun G., Kaplan A.A., Coskun H.S., Erdogan F., Kaplan S.
Cells Tissues Organs
Sumarawati T., Putra A., Jessika C.
Biosaintifika
Putra A., Mulyani S.P., Rahardja C.K.
Biosaintifika
Dirja B.T., Putra A., Amalina N.D., Wardoyo E.H.
Trends in Sciences
Prayitno A., Soetrisno, Laqif A., Kusumo M.H.B.
Archives of Medical Science
