The Role of Mesenchymal Stem Cell Secretome in the Inflammatory Mediators and the Survival Rate of Rat Model of Sepsis
Bisri T., Farhat F., Ilyas S., Munir D., Jusuf N.K., Sari M.I., Putra A., Amin M.M., Rusda M., Muhar A.M.
Abstract
In sepsis, simultaneously elevated levels of pro-inflammatory cytokines and interleukin (IL)-10 indicate immune response dysregulation, increasing the mortality of the host. As mesenchymal stem cell (MSC) secretome is known to have immunomodulatory effects, we aim to assess the role of MSC secretome in the inflammatory mediators (NF-κB p65 and p50, TNF-α, IL-10) and the survival rate of a rat model of sepsis. In this study, forty-eight male Rattus norvegicus rats were divided into one sham group and three groups with sepsis induction: the control group and the sepsis-induced rat groups treated with 150 μL (T1) and 300 μL (T2) of secretome. The survival rate was observed per 6 h for 48 h and plotted using the Kaplan–Meier method. Compared to the control group, T2 showed a significant decrease in the relative expression of NF-κB and the serum TNF-α level, and a significant increase in the serum IL-10 level. Meanwhile, T1 showed a significant decrease in the serum TNF-α level compared to the control group. The Kaplan–Meier Log Rank test did not show significance in the distribution of survival between T1, T2, and the control group. However, from the 18th to the 36th hour, the survival rate of T2 was lower than the survival rate of the control group and T1, with a noticeable difference between T2 and the control group, as well as T1 at the 36th hour. At the 42nd hour, the survival rate of T2 was the same as the control group and remained lower than T1. In conclusion, MSC secretome regulated the inflammatory mediators in rat model of sepsis, with a dose of 150 μL being more effective.
Research on the effect of cytokine concentration on the immune level and survival conditions of elderly patients with sepsis
Li X., Yan B.
Increased Production of Interleukin-10 and Tumor Necrosis Factor-Alpha in Stimulated Peripheral Blood Mononuclear Cells after Inhibition of S100A12
Chu C.-M., Hu H.-C., Hu H.-C., Kao K.-C., Kao K.-C., Leu S.-W., Leu S.-W., Li L.-F., Li L.-F., Lin S.-W., Lin S.-W., Liu P.-H., Liu P.-H., Wu H.-P., Wu H.-P., Yu C.-C., Yu C.-C.
Amitriptyline Treatment Mitigates Sepsis-Induced Tumor Necrosis Factor Expression and Coagulopathy
Beckmann N., Caldwell C.C., Goetzman H.S., Goodman M.D., Gulbins E., Gulbins E., Kim Y., Nomellini V., Veile R.E., Winer L.K., Xia B.T.
Potential Targets to Mitigate Trauma- or Sepsis-Induced Immune Suppression
Beckmann N., Bergmann C.B., Caldwell C.C., Caldwell C.C., Crisologo P.A., Hanschen M., Salyer C.E.
Increased PD-1 expression and altered T cell repertoire diversity predict mortality in patients with septic shock: A preliminary study
Aoki R., Hashiba M., Kajita Y., Kano H., Takeyama N., Terajima T., Tomino A., Tsuda M.
Sepsis-induced t cell immunoparalysis: The ins and outs of impaired T cell immunity
Badovinac V.P., Badovinac V.P., Griffith T.S., Griffith T.S., Griffith T.S., Jensen I.J., Sjaastad F.V.
Sepsis-induced immunosuppression: mechanisms, diagnosis and current treatment options
Cai Q.-L., Cao J., Deng J., Fang X.-M., Gao C., Guan C.-X., Huang S.-Y., Jiang J.-X., Jin S.-W., Li L., Liu D., Sun J.-H., Xu F., Zeng L., Zhang H.-C., Zhou Y.
Immunotherapy of Cancer by Targeting Regulatory T cells
Chen B.-J., Wu G.-Q., Zhang D.-H., Zhao J.-W., Zheng A.-H.
Sepsis: The evolution in definition, pathophysiology, and management
Dhamoon A.S., Gyawali B., Ramakrishna K.
NF-κB signaling in macrophages: Dynamics, crosstalk, and signal integration
Dorrington M.G., Fraser I.D.C.
Darlan D.M., Mangunatmadja I., Putra A., Japardi I., Rusda M., Daulay R.S., Sofyani S., Silvana S., Andreas Y.
Baghdad Science Journal
Krasilnikova I.A., Makarov A.V., Frolov D.A., Salikhova D.I., Shedenkova M.O., Savostyanov K.V., Sudina A.K., Nekrasova A.A., Belousova E.V., Nefedova Z.A., Bakaeva Z.V., Goldshtein D.V., Surin A.M., Fatkhudinov T.K.
Frontiers in Cellular Neuroscience
Jiang Y., Jiang B., Zeng Q., Song Y.
Bioengineering
