Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX2, and Healing Histology Score in Rotator Cuff Tear Rats
Purba A., Putra A., Dias Ismiarto Y., Hidayah N., Fredianto M., Herman H.
Abstract
Objectives: In order to treat a rat model of rotator cuff rupture, this work concentrated on the expression of TNMD and RUNX2, followed by rotator cuff repair and secretome-hMSCs. Methods: A total of thirty 10-weeks-old male Sprague–Dawley rats were separated into five groups randomly, RC on week 0, lesion treated with a rotator cuff repair and saline (RC + NaCl group, n = 6) for 2 and 8 weeks, and lesion treated with a rotator cuff repair and secretome-hMSCs (RC + secretome-hMSC group, n = 6) for 2 and 8 weeks. The supraspinatus and infraspinatus muscle–tendon units were obtained for histological and biomechanical investigation at 0, 2 and 8 weeks following injury. Results: The findings showed that, in comparison with the RC + NaCl group, secretome-hMSCs significantly improved tendon repair by upregulating TNMD and RUNX2 expression and histology score. Conclusion: Combining Secretome-hypoxia MSCs with RC healing may help rats with rotator cuff tears.
Osteoinductive Properties of Secretome of Human Mesenchymal Stem Cells, Obtained with Automatic Cell Culture System
Aleksandrova S.A., Blinova M.I., Khotin M.G., Mikhailova N.A., Nadezhdin S.V., Nashchekina Y.A., Pokrovskaya L.A., Savchenko R.R., Vasiliev S.A.
Carnosine impedes pdgf-stimulated proliferation and migration of vascular smooth muscle cells in vitro and sprout outgrowth ex vivo
Hwang B., Kim J.T., Kim W.-J., Moon S.-K., Park S.L., Song J.-H.
Mechanical tension promotes the osteogenic differentiation of rat tendon-derived stem cells through the Wnt5a/Wnt5b/JNK signaling pathway
Chen W., Liu X., Tang K., Zhang J., Zhou Y.
MIR-488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2
Chen D., Hou Q., Huang Y., Jiang T., Luo Y., Su H.
Biological characteristics and osteogenic differentiation of ovine bone marrow derived mesenchymal stem cells stimulated with FGF-2 and BMP-2
Antonczyk A., Buczak K., Gromolak S., Kielbowicz Z., Klimczak A., Krawczenko A.
Adipose-derived stem cell exosomes facilitate rotator cuff repair by mediating tendon-derived stem cells
Fan A., Fan A., Fu G., Fu G., Fu G., Lu L., Lu L., Pan Z., Pan Z., Yin F., Yin F.
Fibroblast Growth Factor 2 Enhances Tendon-to-Bone Healing in a Rat Rotator Cuff Repair of Chronic Tears
Arimura H., Hiraki Y., Ide J., Ideo K., Karasugi T., Mizuta H., Nakamura E., Shukunami C., Tokunaga T., Yonemitsu R.
The mesenchymal stem cell secretome: A new paradigm towards cell-free therapeutic mode in regenerative medicine
K. R., Kandoi S., L. P.K., Misra R., S. V., Verma R.S.
Secretome studies of mesenchymal stromal cells (MSCs) isolated from three tissue sources reveal subtle differences in potency
Bhonde R., Konala V.B.R., Konala V.B.R., Pal R.
Stem Cell Secretome and Its Effect on Cellular Mechanisms Relevant to Wound Healing
Hong I.-S., Jun H.-S., Kim J.-W., Lee H.-Y., Park S.-R., Roh J.Y., Hong I.-S., Jun H.-S., Kim J.-W., Lee H.-Y., Park S.-R., Roh J.Y.
