Abstract

The study aimed to investigate the impact of UV-B irradiated oyster mushroom powder (referred to as Oyster-D) on blood sugar and TNF-α levels in type 2 diabetic rats induced with streptozotocin (STZ) over 28 days. Twenty-five male rats were divided into five groups: (A) negative control with STZ induction, (B), (C), (D) treated with Oyster-D at doses of 2000 IU, 4000 IU, 8000 IU, and (E) a normal group without STZ induction. Following treatment, blood samples were collected to analyze fasting plasma glucose (FPG) levels using the GOD-PAP method, while TNF-α levels were assessed using the ELISA method. Statistical analysis included One-Way ANOVA and Post Hoc LSD for mean FPG level differences and Kruskal Wallis and Mann-Whitney tests for mean TNF-α levels. The results revealed a significant reduction in mean FPG levels after treatment in the negative control compared to the Oyster-D treatment groups at doses of 2000 IU, 4000 IU, and 8000 IU (p<0.05). Additionally, a notable increase in mean TNF-α levels was observed in the negative control group relative to the Oyster-D treatment group at a dose of 8000 IU (p<0.05). Overall, Oyster-D exhibited potential in reducing blood sugar levels and elevating TNF-α levels in male Wistar rats induced with STZ, suggesting its possible utility in managing hyperglycemia and TNF-α expression in diabetes.