Topical gel of mesenchymal stem cells-conditioned medium under TNF-α precondition accelerates wound closure healing in full-thickness skin defect animal model
Dirja B.T., Putra A., Muhar A.M., Ibrahim S., Pasongka Z., Kuntardjo N., Tunru I.S.
Abstract
Mesenchymal Stem Cells (MSCs) under TNF-α stimulation (MSC-CM-T) can release numerous trophic and survival molecules that have a promising prospect in wound healing acceleration. However, the effective levels of MSC-CM-T in topical gel preparation to accelerate wound healing should be further explored. The aim of this study was to investigate the effects of MSC-CM-T in topical gel preparation in accelerating optimal wound healing through analyzing PDGF levels, wound closure rate percentages, and fibroblast density appearances. Twenty-four male Wistar rats were performed a full-thickness excision. The group studies were randomly assigned into four subgroups: control gel, control medium, and two treatment groups: MSC-CM-T topical gel at doses of 100 μL and 200 μL (T1 and T2, respectively). Wound closure rates were measured by standard caliper, platelet-derived growth factor (PDGF) levels were analyzed using ELISA on days 3 and 6, whereas the fibroblast density appearances were determined using hematoxylin-eosin staining. This study found a significant increase in PDGF levels in all treatment groups on days 3 and 6. These findings were in line with the increase of wound closure rates in all treatment groups on day 6, in which the high dose of MSC-CM-T was more effective in ini-tiating the increase of wound closure rate. We also found the fibroblast density appearances on day 6 in the T2 group. We conclude that the topical gel of MSC-CM-T is more effective in accelerating wound closure healing through increasing PDGF levels and wound closure percentages and fibroblast density appearances in the skin defect animal models.
The effect of mesenchymal stem cells for the reduction of liver fibrosis through platelet derived growth factorâ regulation in rats
Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T.
Intravenous Umbilical Cord-derived Mesenchymal Stem Cells Transplantation Regulates Hyaluronic Acid and Interleukin-10 Secretion Producing Low-grade Liver Fibrosis in Experimental Rat
Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T.
Topical application of recombinant platelet-derived growth factor increases the rate of healing and the level of proteins that regulate this response
Blalock T.D., Blalock T.D., Chin G., Gandhi K., Gowda S., Mast B.A., Mast B.A., Schultz G.S., Schultz G.S., Weinstein D.A.
Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells
Choi J.W., Han J.Y., Jeong J.-W., Kim S., Kim T.M., Kim Y.M., Park T.S., Seo H.W., Song G.
Intravenous Administration is the Best Route of Mesenchymal Stem Cells Migration in Improving Liver Function Enzyme of Acute Liver Failure
Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I., Alif I., Antari A.D., Aryanti R., Darlan D.M., Hayuningtyas F., Makarim F.R., Putra A., Rosdiana I., Wijaya I.
Mesenchymal stem cell biodistribution, migration, and homing in vivo
Bonnet D., Dominici M., Krampera M., Phinney D.G., Zhao W.
Effectiveness of mesenchymal stem cell-conditioned medium in bone regeneration in animal and human models: a systematic review and meta-analysis
Benavides-Castellanos M.P., Garzon-Orjuela N., Linero I.
The effects of cytokines in adipose stem cell-conditioned medium on the migration and proliferation of skin fibroblasts in vitro
Chen L., Gong N., Hu L., Liu J., Zhao J.
Clinical application of growth factors and cytokines in wound healing
Barrientos S., Brem H., Stojadinovic O., Tomic-Canic M.
Platelet-derived growth factor-AA is a substantial factor in the ability of adipose-derived stem cells and endothelial progenitor cells to enhance wound healing
Chan J.Y.-H., Chan J.Y.-H., Chen W.-L., Chen W.-L., Huang S.-M., Wu L.-W., Wu L.-W., Chan J.Y.-H., Chan J.Y.-H., Chen W.-L., Chen W.-L., Huang S.-M., Wu L.-W., Wu L.-W.
Ramirez M.A., Calle A.
International Journal of Molecular Sciences
Krizanova O., Hokynkova A., Penesova A., Sokol J., Babula P., Samadian A.
Frontiers in Physiology
Rinendyaputri R., Rilianawati, Dwicantika A.
Journal of Multidisciplinary Healthcare
Azizidoost S., Farzaneh M., Anbiyaiee A.
Current Signal Transduction Therapy
Mohammadimehr M., Saeedi M., Ahanjan M., Mellati A., Sheidaei S., Teymouri H.
Iranian Journal of Microbiology
Babenko N., Kumetchko M., Litvinova O., Komarchuk I., Pavlov S.
Polski Merkuriusz Lekarski
Mitra Mazumder P., Chowdhury A.
Inflammopharmacology
Perrie Y., Hammad A., Alaqabani H., Abosnwber Y.
International Journal of Pharmaceutics
Abdulla M.A., H. Al-Qaisi T.S., Hawwal M.F., Awad M., Jabbar A.A.J., Hassan R.R., Raouf M.M.H.M., Saleh M.I., AbdulSamad Ismail P., Mothana R.A.
Journal of Molecular Histology
