Umbilical Cord-Derived Mesenchymal Stem Cells Improve TGF-β, α-SMA and Collagen on Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats
Ichwan M., Ilyas S., Rasyid N., Munir D., Warli S.M., Siregar K.B., Putra A., Mukti A.I.
Abstract
Background: A Erectile dysfunction (ED) is one of the well-known comorbidities in males with diabetes mellitus (DM), whose pathogenesis might be induced by dysregulation of corpus cavernosum smooth muscle cells. UC-MSCs are multipotent cells that attract considerable interest due to immunoregulatory properties and might be a potential strategy to regulate and recover the functional cells and tissues, including tissue improvement in DMED. Objective: This study aims to determine the efficacy of UC-MSCs in improving the erectile function of DMED rats through analyzing the expression of TGF-β, α-SMA, and collagen. Methods: Total number of 30 male Sprague-Dawley rats (6 to 8 weeks old) were randomly divided into four groups (negative control group, positive control group, T1 group, and T2 group). After 16 h fast, 24 rats were randomly selected and intraperitoneally injected with streptozotocin to induce DM. At 8 weeks after STZ injection, rats with DMED were identified by unresponsive erectile stimulation within 30 min. PC group received 500 μL; T1 rats treated with 500 μL PBS containing 1x106 UC-MSCs; T2 rats treated with 500 μL PBS containing 3x106 UC-MSCs. After MSCs treatment, the rats were sacrificed and the corpus cavernosum tissues were prepared for histological observations. Results: This study resulted in the administration of UC-MSCs could downregulate the expression of TGF-β, α-SMA, and collagen leading to the improvement of DMED. Conclusion: UC-MSCs improve the expression of TGF-β, α-SMA, and collagen on erectile dysfunction in streptozotocin-in-duced diabetic rats.
Collagen structure and stability
Raines R.T., Shoulders M.D., Raines R.T., Shoulders M.D.
AT-MSCs antifibrotic activity is improved by Eugenol through modulation of TGF-β/Smad signaling pathway in rats
Eldien H.M.S., Eldien H.M.S., Fathy M., Fathy M., Okabe M., Yoshida T.
New insights into TGF-β/Smad signaling in tissue fibrosis
Cao G., Chen D.-Q., Feng Y.-L., Hu H.-H., Vaziri N.D., Wang Y.-N., Zhao Y.-Y., Cao G., Chen D.-Q., Feng Y.-L., Hu H.-H., Vaziri N.D., Wang Y.-N., Zhao Y.-Y.
Transforming growth factor beta 1 increases collagen content, and stimulates procollagen I and tissue inhibitor of metalloproteinase-1 production of dental pulp cells: Role of MEK/ERK and activin receptor-like kinase-5/Smad signaling
Chan C.-P., Chang H.-H., Chang M.-C., Chang M.-C., Jeng J.-H., Jeng P.-Y., Jeng P.-Y., Kuo H.-Y., Liao W.-C., Lin P.-S., Liu H.-C., Yeh C.-Y., Yeung S.-Y., Chan C.-P., Chang H.-H., Chang M.-C., Chang M.-C., Jeng J.-H., Jeng P.-Y., Jeng P.-Y., Kuo H.-Y., Liao W.-C., Lin P.-S., Liu H.-C., Yeh C.-Y., Yeung S.-Y., Chan C.-P., Chang H.-H., Chang M.-C., Chang M.-C., Jeng J.-H., Jeng P.-Y., Jeng P.-Y., Kuo H.-Y., Liao W.-C., Lin P.-S., Liu H.-C., Yeh C.-Y., Yeung S.-Y., Chan C.-P., Chang H.-H., Chang M.-C., Jeng J.-H., Jeng P.-Y., Kuo H.-Y., Liao W.-C., Lin P.-S., Liu H.-C., Yeh C.-Y., Yeung S.-Y.
Erectile Dysfunction and Peyronie's Disease: Genetic Diseases?
Christensen M.B., Hotaling J.M., Pastuszak A.W., Patel D.P.
Interleukin 10 gene-modified bone marrow-derived dendritic cells attenuate liver fibrosis in mice by inducing regulatory T cells and inhibiting the TGF-β/Smad signaling pathway
Chen Y., Guo Y., Li S., Liu M., Lu M., Tang X., Xu Y., Yang M., Zhang S., Chen Y., Guo Y., Li S., Liu M., Lu M., Tang X., Xu Y., Yang M., Zhang S.
Therapeutic effect of interleukin-10 in keloid fibroblasts by suppression of TGF-β/Smad pathway
Ge P., Huang G.-B., Shi C.-K., Zhao Y.-P.
Fibroblast fate regulation by time dependent TGF-β1 and IL-10 stimulation in biomimetic 3D matrices
Anderegg U., Ansorge M., Chkolnikov M., Pompe T., Sapudom J., Wu X., Anderegg U., Ansorge M., Chkolnikov M., Pompe T., Sapudom J., Wu X.
Suppression of TGF-beta activity with remobilization attenuates immobilization-induced joint contracture in rats
Li F., Mao D., Mi J., Pan X., Rui Y.
Transforming growth factor-β (TGF-β)-induced up-regulation of TGF-β receptors at the cell surface amplifies the TGF-β response
Derynck R., Duan D.
Chung E., Wang H.
Expert Opinion on Drug Discovery
Lopez-Martin L., Chavez-Roa C., Garcia-Arranz M., Simon-Rodriguez C., Maqueda-Arellano J., Osorio-Ospina F., Perez-Aizpurua X., Tufet i Jaumot J.J., Ruiz de Castroviejo-Blanco J., Alonso-Roman J., Gomez-Jordana B., Garranzo-Ibarrola M., Garcia-Cardoso J.V., Gonzalez-Enguita C.
Life
Ryu J.-K., Yin G.N., Chung D.Y.
Investigative and Clinical Urology
Gu J., Pang K., Shi Z.-D., Zhu Z.-B., Liu Y., Li Z.-B., Wang J., Wang W., Wang J.-K., Han C.-H.
Biodrugs
Pilehvar Y., Lestari S.W., Margiana R., I'tishom R., Supardi S., Amalia F.L.
Asian Journal of Urology
Prayitno A., Soetrisno, Laqif A., Kusumo M.H.B.
Archives of Medical Science
Muzammil K., Waggiallah H.A., Patel A.A., Shafie A., Al Abdulmonem W., Mohamed A.H., Tayeb F.J., Ahmad I., AlShahrani A.M., Ali S.A.-J., Sheweita S.A.
Stem Cell Research and Therapy
