Hypoxia-mesenchymal stem cells inhibit intra-peritoneal adhesions formation by upregulation of the il-10 expression
Munir D., Warli S.M., Putra A., Muhar A.M.
Abstract
BACKGROUND: Intra-peritoneal adhesions (IPAs) common occurre in post abdominal surgical. Athough many methods have been developed for controlling IPAs, including mesenchymal stem cells (MSCs) application, however, there is none completely preventing in due to the mesothelial structure may promote the prolonged inflammations leading. Nevertheless hypoxia-MSCs (H-MSCs) have more potent in controlling the inflammation than normoxia-MSCs (N-MSCs) by releasing several anti-inflamation particularly IL-10, however the H-MSCs application to inhibit IPAs remain unclear. AIM: The aim of this study was to investigate the effectiveness of H-MSCs in preventing the AIPs event by releasing IL-10 on the ileum abrasion sutured omental patch as the animal model of peritoneal adhesion. METHODS: Using 24 IPAs animal model were randomly divided into 4 groups: Sham (Sh), Control (C), H-MSCs at high dose (T1) and H-MSCs at low dose (T2). H-MSCs were incubated under hypoxic conditions (5% O<inf>2</inf>), 37°C and 5% CO<inf>2</inf> for 24 hours. The expression level of IL-10 was performed using RT-PCR analysis. The macroscopic appearance of IPAs was evaluated using Nair’s scale base on the absence/presence of adhesion, whereas the microscopic by Zuhlke’s scale at Hematoxylin and eosin (H&E) staining. RESULTS: This study showed a significanly increase in IL-10 expression (p < 0.05) at all T groups. In line with this, we also found a significant difference in IPAs between T groups and Control as well as a Sham (p < 0.05) either in the macroscopic or microscopic analysis. CONCLUSION: H-MSCs has a robust ability in inhibiting severe IPAs characterized by the decreased of adhesion formation and the enhanced expression of IL-10.
TGF-β and the tissue microenvironment: Relevance in fibrosis and cancer
Caballero-Diaz D., Caja L., Dituri F., Fabregat I., Giannelli G., Mancarella S., Moustakas A.
A simple method to isolate and expand human umbilical cord derived mesenchymal stem cells: Using explant method and umbilical cord blood serum
Aljamali M., Hassan G., Kasem I., Soukkarieh C.
Standardised models for inducing experimental peritoneal adhesions in female rats
Brochhausen C., Kraemer B., Rajab T.K., Rothmund R., Wallwiener C., Wallwiener M.
Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of plc-γ and potential clinical implications
Harding K.G., Jiang W.G., Ruge F., Sanders A.J.
Interleukin-6 stimulates Akt and p38 MAPK phosphorylation and fibroblast migration in non-diabetic but not diabetic mice
Hayashi A., Kado M., Kanazawa S., Luo L., Mizuno H., Okada K., Shen T.N.-Y., Tanaka R.
Pathophysiology of acute wound healing
Chen J., Kirsner R., Li J.
Critical Role of Transforming Growth Factor Beta in Different Phases of Wound Healing
Farrokhi A., Ghahary A., Maharlooei M.K., Pakyari M.
Suppression of antigen presentation by IL-10
Mittal S.K., Roche P.A.
Pharmacological Mobilization and Recruitment of Stem Cells in Rats Stops Abdominal Adhesions After Laparotomy
Ahmadi A.R., Burdick J., Cameron A.M., Chen M., Iwasaki K., Katsumata K., Qi L., Sun Z., Tsuchida A., Wang W.
Mesothelial cells in tissue repair and fibrosis
Birnie K., Herrick S.E., Lansley S., Lim C.-B., Mutsaers S.E., Prele C.M., Birnie K., Herrick S.E., Lansley S., Lim C.-B., Lim C.-B., Mutsaers S.E., Mutsaers S.E., Prele C.M., Prele C.M.
