Revealing the decrease of indoleamine 2,3-dioxygenase as a major constituent for B cells survival post-mesenchymal stem cells co-cultured with peripheral blood mononuclear cell (PBMC) of systemic lupus erythematosus (SLE) patients
Darlan D.M., Munir D., Jusuf N.K., Putra I.B., Putra A., Amalina N.D., Alif I.
Abstract
Aim Mesenchymal stem cells (MSCs) have potent immunosuppre-ssive properties to control systemic lupus erythematosus (SLE) disease by inhibiting indoleamine 2,3-dioxygenase (IDO), and increasing regulatory T cells (Treg) to control innate and adaptive immune cells. However, the interaction and mechanism regarding IDO and B cells in the co-culture of MSC and SLE peripheral blood mononuclear cell (PBMCs) remain unclear. This study aimed to investigate the effects of MSCs in controlling B cells through IDO expression in PBMC of SLE patients. Methods This study used a post-test control group design. MSCs were obtained from human umbilical cord blood and characterized according to their surface antigen expression and multilineage differentiation capacities. PBMCs isolated from SLE patients were divided into five groups: sham, control, and three treatment gro-ups. The treatment groups were treated by co-culturing MSCs to PBMCs with a ratio of 1:10, 1:25, and 1:40 for 72 h incubation. The B cell levels were analysed by flow cytometry with cytometric bead array (CBA) and the IDO levels were determined by ELISA. Results The percentages of B cells decreased significantly in groups treated by dose-dependent MSCs, particularly in T1 and T2 groups. These findings were aligned with the significant decrease of the IDO level. Conclusion MSCs control B cells-mediated by a decrease of IDO in PBMC of SLE patients.
In vitro regulation of IL-6 and TGF-ß by mesenchymal stem cells in systemic lupus erythematosus patients
Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A., Alif I., Ikhsan R., Karmila Jusuf N., Masyithah Darlan D., Munir D., Putra A.
The therapeutic efficacy of mesenchymal stromal cells on experimental colitis was improved by the IFN-γ and poly(I:C) priming through promoting the expression of indoleamine 2,3-dioxygenase
Kim B.-S., Kim T.W., Lim J.-Y., Min C.-K., Park G., Ryu D.-B., Kim B.-S., Kim T.W., Lim J.-Y., Min C.-K., Park G., Ryu D.-B.
IDO expression by human B lymphocytes in response to T lymphocyte stimuli and TLR engagement is biologically inactive
Duvignaud J.-B., Duvignaud J.-B., Godin-Ethier J., Hanafi L.-A., Lapointe R., Leclerc D.
Indoleamine 2,3-dioxygenase specific, cytotoxic T cells as immune regulators
Andersen M.H., Andersen M.H., Hadrup S.R., Hjortso M.C., Sorensen R.B., Straten P.T., Svane I.M.
Intracellular B Lymphocyte Signalling and the Regulation of Humoral Immunity and Autoimmunity
Abraham D.J., Bystrom J., Isenberg D.A., Mageed R.A., Ong V.H., Renaudineau Y., Taher T.E.
Cutting edge: Regulatory T cells directly suppress B cells in systemic lupus erythematosus
Hahn B.H., Iikuni N., La Cava A., Lourenco E.V., Hahn B.H., Iikuni N., La Cava A., Lourenco E.V.
Isolation, differentiation, and characterization of mesenchymal stem cells from human bone marrow
Assadzadeh-Aghdaei H., Baghaei K., Hashemi S.M., Rad A.A., Sharifian A., Tokhanbigli S., Zali M.R., Assadzadeh-Aghdaei H., Baghaei K., Hashemi S.M., Rad A.A., Sharifian A., Tokhanbigli S., Zali M.R., Assadzadeh-Aghdaei H., Baghaei K., Hashemi S.M., Rad A.A., Sharifian A., Tokhanbigli S., Zali M.R.
The role of mesenchymal stem cells in decreasing interleukin-12 human systemic lupus erythematosus
Alif I., Darlan D.M., Lubis R.R., Munir D., Putra A., Alif I., Darlan D.M., Lubis R.R., Munir D., Putra A., Alif I., Darlan D.M., Lubis R.R., Munir D., Putra A., Alif I., Darlan D.M., Lubis R.R., Munir D., Putra A.
MSCs-released TGFβ1 generate CD4+CD25+Foxp3+ in T-reg cells of human SLE PBMC
Darlan DM, Jusuf NK., Munir D, Putra A, Darlan DM, Jusuf NK., Munir D, Putra A, Darlan DM, Jusuf NK., Munir D, Putra A, Darlan DM, Jusuf NK., Munir D, Putra A
Interaction between mesenchymal stem cells and B-cells
Cen P., Chen J., Fan L., Hu C., Li L., Wang J., Cen P., Chen J., Fan L., Hu C., Li L., Wang J., Cen P., Chen J., Fan L., Hu C., Li L., Wang J.
Sumarawati T., Putra A., Trisnadi S., Amalina N.D., Setiawan E., Hermansyah D., Angelina J.
Medicinski Glasnik
Signal Transduction and Targeted Therapy
Putra A., Amalina N.D., Muhar A.M., Alif I., Hermansyah D., Hidayah N., Makharim F.
Indonesian Journal of Biotechnology
Putra A., Wibowo J.W., Amalina N.D., Priyantini S., Sari A.R.
Medicinski Glasnik
Ismiarto Y.D., Putra A., Amalina N.D., Alif I., Nazar M.A., Fredianto M., Herman H.
Medicinski Glasnik
Suzery M., Cahyono B., Amalina N.D.
Medicinski Glasnik
Putra A., Wibowo J.W., Amalina N.D., Irawan R.C.S., Utami A.
Medicinski Glasnik
Margiana R.
Cell Biochemistry and Function
Bukhari A., Hardjo M., Patellongi I., Achmad M.H., Putra A., Daud N.A.S., Amansyah F., Parewangi L., Budu B., Massi M.N.
Pakistan Journal of Biological Sciences
Wisnu Prajoko Y., Putra A., Prasetio A., Dina Amalina N.
Medicinski Glasnik
