Secretome of Hypoxic Mesenchymal Stem Cells Improves Fluconazole-Induced Alopecia in Rats via Immunoregulatory Modulation of IL-15 and IFN-γ
Putra A., Mulyani S.P., Rahardja C.K.
Abstract
Increased IL-15 and IFN-γ characterize fluconazole-related alopecia (FRA). The hypoxia mesenchymal stem cell (hMSC) secretome has anti-inflammatory capabilities that can potentially be used as alopecia therapy. Therefore, the study aims to determine the effect of topical administration of hMSCs secretome gel on reducing IL-15 and IFN-γ gene expression and baldness in male Wistar rats, a model of FRA. MSC was collected from rat umbilical cord, cultured under hypoxia for 24 hours, and yielded a sterile secretome formulated into a water-based gel ointment for treatment. A total of 24 male Wistar rats were randomly divided into four groups: K1 for health controls with placebo administration only; K2 for negative control which contain FRA rats by applying fluconazole from day 7<sup>th</sup> to 14<sup>th</sup> and continued placebo administration until day 29<sup>th</sup>; treatments were conducted for FRA rat groups treated with 200 mg/day of topical gel contained with 10% of hMSCs secretome for K3 and 20% of hMSCs secretome for K4. Observations were made to analyze hair growth and IL-15 and INF-γ mRNA expression using qPCR. The analysis showed a significant decrease in IL-15 and IFN-γ mRNA expression (p ≤ 0.001) and a reduction in baldness of up to 60% after topical hMSCs secretome gel administration. The prominent result was that the topical gel contained 20% of hMSCs secretome. Based on research results, a topical gel dose containing 20% hMSC secretome had the best effect on improving the condition of FRA. This research may help optimize doses and treatment methods in hMSC secretome therapy.
Platelet sonicates activate hair follicle stem cells and mediate enhanced hair follicle regeneration
Chen Y., Cheng B., Cheng B., Fang K., Kong D., Lei X., Liu Cheng H., Mo M., Pang M., Pang M., Tian R., Wu Y., Yang G., Zhu M., Zhu M., Zhu M., Chen Y., Cheng B., Cheng B., Fang K., Kong D., Lei X., Liu Cheng H., Mo M., Pang M., Pang M., Tian R., Wu Y., Yang G., Zhu M., Zhu M., Zhu M.
Current advances in stem cell-based therapies for hair regeneration
Bian Q., Gao J.-Q., Gao J.-Q., Gao J.-Q., Yuan A.-R., Bian Q., Gao J.-Q., Gao J.-Q., Gao J.-Q., Yuan A.-R.
Hypoxic Preconditioning Enhances the Efficacy of Mesenchymal Stem Cells-Derived Conditioned Medium in Switching Microglia toward Anti-inflammatory Polarization in Ischemia/Reperfusion
Chen Y., He X., Lei Y., Li X., Li Y., Lin L., Qu G., Wang H., Xie X., Xu Z., Yu H., Yu H.
Alopecia areata is driven by cytotoxic T lymphocytes and is reversed by JAK inhibition
Cerise J.E., Christiano A.M., Clynes R., Dai Z., De Jong A., Destefano G.M., Gong W., Harel S., Higgins C.A., Jabbari A., MacKay-Wiggan J., Petukhova L., Rothman L., Singh P., Xing L.
In Situ Precision Cell Electrospinning as an Efficient Stem Cell Delivery Approach for Cutaneous Wound Healing
Chen H., Chen H., Chen Y., Duan Y., Liao P., Liu S., Moroni L., Wang F., Wang N., Wen Z., Wu H., Zeng W., Zhang M.
The potential of regulatory T cell-based therapies for alopecia areata
Guan C., Song X., Wan S., Xie B., Xu W.
Regulatory T cells in autoimmune skin diseases
Ujiie H.
Resident human dermal γδT-cells operate as stress-sentinels: Lessons from the hair follicle
Almeida L., Bertolini M., Cheret J., Funk W., Gherardini J., Gherardini J., Gilhar A., Gross C.C., Kanekura T., Pappelbaum K., Paus R., Paus R., Paus R., Rossi A., Schulte-Mecklenbeck A., Strbo N., Uchida Y.
Evaluation of serum levels of IL-6, IL-10, and TNF-α in alopecia areata patients: A systematic review and meta-analysis
Dalvand E., Eftekharian M.M., Farsiabi R., Khazaei S., Moghimi H., Torkestani S.
Examination of fluconazole-induced alopecia in an animal model and human cohort
Aaron P.A., Affolter V.K., Dennis M., Everett A.D., Fedor D.M., Gelli A., Katarina Varjonen E., Krois C.R., McHardy I., Napoli J.L., Sharon V.R., Singapuri A., Thompson G.R., Thompson G.R., White S.D., Wiederhold N.P., Yoo H.S., Aaron P.A., Affolter V.K., Dennis M., Everett A.D., Fedor D.M., Gelli A., Katarina Varjonen E., Krois C.R., McHardy I., Napoli J.L., Sharon V.R., Singapuri A., Thompson G.R., Thompson G.R., White S.D., Wiederhold N.P., Yoo H.S.
Putra A., Sarosa H., Trisnadi S., Amalina N.D., Mulyani S.P., Ibrahim S., Dewi A.M., Chodijah C.
Biosaintifika
Alaydrus A.A., Alaydrus H.S.
Trends in Sciences
