Enhanced Third Degree Burn Wound Healing by Hypoxic Mesenchymal Stem Cells' Secretome: IL-10 Upregulation and TNF-α/PGE2 Suppression
Dirja B.T., Putra A., Amalina N.D., Wardoyo E.H.
Abstract
Background: Severe third-degree burns pose a notable obstacle in clinical settings, frequently leading to hindered healing of wounds and the development of scars. In this research, we explored how the secretome of hypoxic mesenchymal stem cells (HS-MSCs) could be improved the healing of burn wounds. Methods: In this study, we used 20 male Wistar rats that induced third degree wound by metal 80 exposure for 10 s. These rats divided into 4 groups: Healthy, control, HS-MSCs 100 µL, and HS-MSCs 200 µL groups. We injected the HS-MSCs, which carry various beneficial molecules like IL-10, TGF-β, PDGF, and VEGF, directly into the skin in a third-degree burn rat model on day 1 after burn induction, and harvest on day 14. Results: Our research discovered that using HS-MSCs resulted in a dosage-related rise in IL-10, an anti-inflammatory cytokine, alongside a notable decrease in TNF-α and PGE2, pro-inflammatory cytokines. These effects quickened the healing process by improving re-epithelialization and minimizing scar formation. Conclusions: Our findings indicate that HS-MSCs show promise as a treatment for severe burns by potentially controlling inflammation and fostering a regenerative healing process. More investigation is needed to understand the exact mechanisms and to explore how HS-MSCs could be applied in clinical treatments.
Secretome of Hypoxia-Preconditioned Mesenchymal Stem Cells Ameliorates Hyperglycemia in Type 2 Diabetes Mellitus Rats
Alif I., Amalina N.D., Irawan R.C.S., Muhar A.M., Prasetio A., Priyantini S., Putra A., Sumarawati T., Trisnadi S., Widyaningsih W., Alif I., Amalina N.D., Amalina N.D., Irawan R.C.S., Muhar A.M., Prasetio A., Priyantini S., Putra A., Putra A., Sumarawati T., Trisnadi S., Widyaningsih W., Alif I., Amalina N.D., Irawan R.C.S., Muhar A.M., Prasetio A., Priyantini S., Putra A., Sumarawati T., Trisnadi S., Widyaningsih W.
Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) inflammation through inhibiting interferon (IFN)-gamma production
Dina Amalina N., Dina Amalina N., Prasetio A., Putra A., Putra A., Wisnu Prajoko Y.
Cell contact, prostaglandin E2 and transforming growth factor beta 1 play non-redundant roles in human mesenchymal stem cell induction of CD4+CD25Highforkhead box P3+ regulatory T cells
Barry F.P., English K., Mahon B.P., Mahon B.P., Murphy M.J., Ryan J.M., Tobin L., Barry F.P., English K., Mahon B.P., Mahon B.P., Murphy M.J., Ryan J.M., Tobin L., Barry F.P., English K., Mahon B.P., Mahon B.P., Murphy M.J., Ryan J.M., Tobin L., Barry F.P., English K., Mahon B.P., Mahon B.P., Murphy M.J., Ryan J.M., Tobin L., Barry F.P., English K., Mahon B.P., Mahon B.P., Murphy M.J., Ryan J.M., Tobin L., Barry F.P., English K., Mahon B.P., Mahon B.P., Murphy M.J., Ryan J.M., Tobin L.
Hypoxia-preconditioned MSCs have superior effect in ameliorating renal function on acute renal failure animal model
Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W., Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W., Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W., Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W., Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W., Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W., Indrayani U.D., Jannah D., Milla M.N., Pertiwi D., Putra A., Sahariyani M., Trisnadi S., Wibowo J.W.
Mesenchymal stem cell therapies for liver cirrhosis: MSCs as "conducting cells" for improvement of liver fibrosis and regeneration
Nojiri S., Ogawa M., Takeuchi S., Terai S., Tsuchiya A., Watanabe T., Yoshida T., Nojiri S., Ogawa M., Takeuchi S., Terai S., Tsuchiya A., Watanabe T., Yoshida T., Nojiri S., Ogawa M., Takeuchi S., Terai S., Tsuchiya A., Watanabe T., Yoshida T.
Systematic review of stem-cell-based therapy of burn wounds: Lessons learned from animal and clinical studies
Fink T., Henriksen J.L., Porsborg S.R., Sorensen N.B., Zachar V.
Hypoxia enhances the wound-healing potential of adipose-derived stem cells in a novel human primarykeratinocyte-based scratch assay
Andersen J.I., Boucher S., Fink T., Ipek H., Kuninger D., Newman R., Pennisi C.P., Riis S., Vemuri M.C., Zachar V., Andersen J.I., Boucher S., Fink T., Ipek H., Ipek H., Kuninger D., Newman R., Pennisi C.P., Riis S., Vemuri M.C., Zachar V., Andersen J.I., Boucher S., Fink T., Ipek H., Ipek H., Kuninger D., Newman R., Pennisi C.P., Riis S., Vemuri M.C., Zachar V.
The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling
Frangogiannis N.G., Humeres C., Shinde A.V., Frangogiannis N.G., Humeres C., Shinde A.V., Frangogiannis N.G., Humeres C., Shinde A.V.
Myofibroblast contraction activates latent TGF-β1 from the extracellular matrix
Hinz B., Meister J.-J., Rifkin D.B., Wipff P.-J., Hinz B., Meister J.-J., Rifkin D.B., Wipff P.-J.
The Role of an IL-10/Hyaluronan Axis in Dermal Wound Healing
Balaji S., Birla R.K., Bollyky P.L., Gilley J., Kaul A., Keswani S.G., Parikh U.M., Singampalli K.L., Singampalli K.L., Singampalli K.L., Wang X., Balaji S., Birla R.K., Bollyky P.L., Gilley J., Kaul A., Keswani S.G., Parikh U.M., Singampalli K.L., Wang X.
Sumarawati T., Putra A., Trisnadi S., Amalina N.D., Setiawan E., Hermansyah D., Angelina J.
Medicinski Glasnik
