Saltwater Fish Powder for Amelogenesis in Zebrafish Larvae (Danio rerio)
Hutami I.R., Suparmi S., Pradopo S., Rochmah Y.S., Ibrahim Z., Christiono S., Nurhapsari A., Indasari N., Yuniar S.N.
Abstract
Many nutrients found in saltwater fish are thought to promote amelogenesis in ameloblast cells during tooth development. The aim of the study is to determine the effect of LC<inf>50</inf> toxicity on saltwater fish powder on zebrafish embryos. The experimental research method was a post-test-only control group design, consisting of eight groups, namely the negative control and the internal group using embryo media, the positive control using 3,4-dichloroanillin, and the treatment group 125 μg/ml, 250 μg/ml, 500 μg/ml, 1000 μg/ml, 2000 μg/ml, and 4000 μg /ml using 384 zebrafish embryos. Saltwater fish powder is diluted and observed for 96 hours, controlled every 24 hours. There were no dead zebrafish embryos from various concentrations, negative control, and internal control. The toxicity test of saltwater fish powder from the lowest concentration of 125 μg/ml to the highest concentration of 4000 μg/ml showed no signs of zebrafish embryo mortality nor developmental abnormalities. It can be concluded that saltwater fish powder is non-toxic.
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