Hypoxia-Induced Mesenchymal Stem Cell Secretome Enhances IL-10 via STAT3 Pathway in a Rat PCOS Model
Lusiana L., Darlan D.M., Trisnadi S., Putra A., Sumarawati T., Chodijah C., Amalina N.D.
Abstract
Polycystic ovary syndrome (PCOS) is a condition characterized by chronic inflammation and hyperandrogenism, commonly leading to infertility. This is often triggered by hyperglycemia and hyperlipidemia, which cause hyperandrogenism. The secretome of mesenchymal stem cells (MSCs) can suppress pro-inflammatory cytokine secretion by enhancing IL-10 expression and inhibiting androgen production in a PCOS rat model. This study aims to investigate the effect of the administration of hypoxic mesenchymal stem cell secretome on IL-10 and STAT3 gene expression in the PCOS model. In vivo experimental research with a post-test-only control group design. The total sample was 24 Wistar female rats, divided into four groups: healthy, negative control (PCOS rats were injected with 0.9% NaCl), T1 (PCOS rats were given secretome at a dose of 200 µl), and T2 (PCOS rats were given Secretome at 400 µl) and were given treatment for 33 days. QRT-PCR determined IL-10 and STAT3 gene expression, and statistical analysis was performed using the One-Way ANOVA test followed by the Post Hoc LSD test. This study demonstrated that the expression of the IL-10 and STAT3 genes significantly differed in the T2 group compared to the negative control. Additionally, there was a notable difference in IL-10 gene expression between groups T2 and T1 compared to the negative control. Furthermore, differences were also observed in the expression of the STAT3 gene in both the T2 and T1 groups. The administration of hypoxic mesenchymal stem cell secretomes increased IL-10 and STAT3 gene expression in PCOS rat models.
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