Abstract

Hyperuricemia is a metabolic condition defined by excessive uric acid levels in the bloodstream. This condition may cause oxidative stress and reactive oxygen species (ROS) generation, overwhelming cellular antioxidant defense systems. Ethanol extract from snake fruit (Salacca zalacca peel (SZP)) contains bioactive compounds with potential antihyperuricemic properties, making it a promising alternative to conventional treatments like allopurinol. The mechanism of action is associated with the level of uric acid, superoxide dismutase (SOD), and malondialdehyde (MDA). Therefore, this research aimed to evaluate the impact of SZP on the level of SOD, MDA, and uric acid in male Wistar rats induced by chicken liver (CL) juice. A total of 30 rats were assigned to five groups, namely group I (control); II (hyperuricemia without any intervention), III (hyperuricemia treated with 1.8 mg/200 g BW allopurinol), IV, and V (hyperuricemia that were administered SZP at doses of 210 mg/kg BW and 420 mg/kg BW, respectively). The results showed significant reductions in uric acid levels (5.78 ± 0.10 mg/dL) and MDA (3.34 ± 0.28 nmol/mL) with the higher doses of SZP treatment (420 mg/kg BW). However, SOD levels increased (73.77 ± 2.00%) compared to the hyperuricemia control. The average SOD and MDA levels exhibited notable variations across all groups (p < 0.05). These findings suggested that ethanol extract from SZP effectively modulates oxidative stress and uric acid metabolism by influencing the serum levels of SOD, MDA, and uric acid and could serve as an alternative treatment for hyperuricemia.