Exploring the α-Amylase Inhibitory Potential of Peronema canescens Jack: An In Vitro and In Silico Study
Susilawati Y., Sumiwi S.A., Suharsanti R., Rahardhian M.R.R., Ramonah D., Aryanti N., Putri C.N.
Abstract
Hyperglycemia in individuals with type 2 diabetes mellitus is primarily driven by the rapid hydrolysis of starch by the enzyme α-amylase in the pancreas and the breakdown of oligosaccharides by α-glucosidase in the intestine. Peronema canescens Jack. (PC) has shown promise as a potential antidiabetic agent. This study aimed to evaluate the total flavonoid, phenolic, and α-amylase inhibitory activity of extracts and fractions derived from PC leaves using both in vitro and in silico approaches. The ethanol extract of PC leaves was fractionated through liquid-liquid extraction using n-hexane, ethyl acetate, and water as solvents. Preliminary phytochemical screening of the extracts and fractions identified the presence of alkaloids, flavonoids, saponins, tannins, and steroids/triterpenoids. The n-hexane fraction exhibited the highest total flavonoid content, averaging 203.37±4.38 mg QE/gram, while the ethyl acetate fraction demonstrated the highest total phenolic content, averaging 147.04±0.79 mg GAE/gram. Furthermore, the ethyl acetate fraction showed the strongest α-amylase inhibitory activity, with an average inhibition rate of 70.38±1.26%. In silico analysis, combined with GC-MS identification, suggested that three compounds, bis(2-ethylhexyl) phthalate, myristyl oleate, and 14 beta H-pregna may contribute to the observed α-amylase inhibitory activity. These findings highlight the potential of PC as a source of natural antidiabetic agents.
pkCSM: Predicting small-molecule pharmacokinetic and toxicity properties using graph-based signatures
Ascher D.B., Blundell T.L., Pires D.E.V., Pires D.E.V.
GC-MS (Gas Chromatography-Mass Spectrometry) analysis of nut grass tuber (Cyperus rotundus L.) methanolic extract
Fatimawali, Hotmian E., Suoth E., Tallei T.
The In Vitro and In Silico Study of α-glucosidase Inhibition by Kombucha Derived from Syzygium polyanthum (Wight) Walp. Leaves
Alfarabi M., Lestari Y., Noviardi H., Yuningtyas S.
Secondary Metabolite Compounds from Alpinia monopleura Extract and Evaluation of Anti-Inflammatory Activity based on In Vitro and In Silico Studies
Arfan, Badia E., Fauziah Y., Fusvita A., Musdalipah, Reymon, Sahidin, Wahyuni, Yodha A.W.M.
No Title
International Diabetes Federation
Webber S.
Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery
Le L., Pham B., Tran N.
Isolation of a Flavone Apigenin and a Steroids Squalene from Peronema canescens Jack Leaves with Anti-Inflammatory Activities
Aini I.P.S., Latief M., Rumaida, Sutrisno, Tarigan I.L.
Metabolite Bioactive Contents of Parkia timoriana (DC) Merr Seed Extracts in Different Solvent Polarities
Handayani D.S., Risqi H.D., Sari F., Sariwati A., Suryanti V.
Integrating molecular docking and molecular dynamics simulations
Caffarena E.R., Ferreira R.S., Santos L.H.S.
