Unlocking the Potential of Stink Bean Peel Extract: A Natural Solution for UVB-Induced Skin Hyperpigmentation
Ustika C.R., Priyantini S., Putra A., Subchan P., Sumarawati T., Amalina N.D.
Abstract
Prolonged exposure to high-intensity UVB radiation can induce the formation of Reactive Oxygen Species (ROS), inhibit alpha-smooth muscle actin (α-SMA), and collagen type 1 expression, leading to skin hyperpigmentation. This study aimed to investigate the effect of topical application of stink bean peel extract gel on the expression of α-SMA and collagen type 1 genes in the skin tissue of hyperpigmentation rats. Stink bean peel extract gel was prepared by mixing ethanol extract of stink bean peel with a gel base (Catechu) to make 10% and 20% formulations. Hyperpigmentation was induced in rats by exposure to UVB radiation (λ302 nm, 390 mj/cm2/day) three times a week for two weeks. The animals were divided into four groups: K1 (healthy rats), K2 (negative control), K3 (10% extract gel, and K4 (20% extract gel). The effect of stink bean peel extract gel treatment on the expression of α-SMA and collagen type 1 genes was analyzed using Real-Time Quantitative Reverse Transcription polymerase chain reaction (qRT-PCR). qRT-PCR analysis indicated a significant (p < 0.05) increase in the expression of α-SMA gene in group K4 with a mean expression ratio of 3.11±1.08 compared to the negative control (0.67±0.17). Collagen type 1 gene expression also increased significantly in K3 and K4 groups with expression ratios of 2.21±0.68, and 2.12±0.73, respectively compared to the negative control (0.51±0.14). Findings from this study has shown that stink bean peel extract has the potential to be used as a protective and therapeutic agent against UVB-induced skin damage.
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