Mesenchymal Stem Cells Suppress TGF-β Release to Decrease α-SMA Expression in Ameliorating CCl4-Induced Liver Fibrosis
Dirja B.T., Putra A., Retnaningsih, Wirastuti K., Muhar A.M., Hermansyah D.
Abstract
Introduction: Liver fibrosis (LF) is the excessive deposition of extracellular matrix (ECM), produced by overactivated hepatic stellate cells, following prolonged transforming growth factor-β (TGF-β) stimulation. The ability of mesenchymal stem cells (MSCs) to improve LF has been reported. However, the mechanisms of MSCs to ameliorate LF through suppressing TGF-β and α-smooth muscle actin (α-SMA) remains unclear. Aim: To investigate the effects of MSCs treatment on suppressing TGF-β levels and decreasing α-SMA expression in an LF model. Methods: In this study, wenty-four male Wistar rats were injected intraperitoneal (IP) with carbon tetrachloride (CCL4), twice weekly, for eight weeks, to induce LF. Rats were randomly assigned to six groups: Sham, Control, Sham-lo, Sham-hi, and MSC-treated groups, at doses of 1 x 10<sup>6</sup>(T1) and 2x10<sup>6</sup>(T2) cells. TGF-β levels were analyzed by enzyme-linked immunosorbent assay (ELISA), whereas α-SMA expression was determined by immunohistochemistry staining. Results: MSCs decreased the expression of TGF-β in T1 and T2 groups on day 3 and 14. The T2 group showed lower TGF-β levels than that in the T1 group. This finding was in line with the observed decrease in α-SMA expression and the number of collagen. Conclusion: MSCs treatment ameliorated LF by suppressing TGF-β production, leading to decreased α-SMA expression in a CCL4-induced LF animal model.
Transplantation of bone marrow-derived mesenchymal stem cells after regional hepatic irradiation ameliorates thioacetamide-induced liver fibrosis in rats
Chai H., Chai H., Chen S.-L., Chen S.-L., Cheng F., Cheng F., Deng L., Dong T.-F., Dong T.-F., Shao C.-H., Shao C.-H., Wang Y., Wang Y., Yu Y.
The therapeutic potential of bone marrow-derived mesenchymal stem cells on hepatic cirrhosis
Dai L.-J., Dai L.-J., Guan L.-X., Li H.Y., Ritchie G., Zhou J.X., Dai L.-J., Dai L.-J., Guan L.-X., Li H.Y., Ritchie G., Zhou J.X.
Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl4 -induced fibrotic liver
Ali G., Masoud M.S.
Hypoxia-mesenchymal stem cells inhibit intra-peritoneal adhesions formation by upregulation of the il-10 expression
Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M.
Fibrosis Related Inflammatory Mediators: Role of the IL-10 Cytokine Family
Beres N.J., Fekete A., Lippai R., Pap D., Szabo A.J., Sziksz E., Sziksz E., Vannay A., Vannay A.
Macrophages during the fibrotic process: M2 as friend and foe
Agudelo J.S.H., Braga T.T., Camara N.O.S., Camara N.O.S., Agudelo J.S.H., Braga T.T., Camara N.O.S., Camara N.O.S.
Alternative activation of macrophages: Mechanism and functions
Gordon S., Martinez F.O.
Mechanisms of CCl4-induced liver fibrosis with combined transcriptomic and proteomic analysis
Chen Q.-L., Dong S., Hu Y.-Y., Li X.-Y., Liu P., Song Y.-N., Su S.-B., Sun Y., Wei B.
The effect of mesenchymal stem cells for the reduction of liver fibrosis through platelet derived growth factorâ regulation in rats
Lindarto D., Putra A., Sembiring R.J., Sungkar T., Lindarto D., Putra A., Sembiring R.J., Sungkar T.
The role of TGF-β receptors in fibrosis
Nakerakanti S., Trojanowska M.
Chintalapani G., Melancon M.P., Heralde F.M., Fowlkes N.W., Barcena A.J.R., Bolinas D.K.M., Bernardino M.R., Lin V., Huang S.Y., Mishra A.
Gels
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