Effectiveness of mesenchymal stem cells and bovine colostrum on decreasing tumor necrosis factor-Α levels and enhancement of macrophages m2 in remnant liver
Prajoko Y.W., Putra A., Gunadi E.E.
Abstract
BACKGROUND: Mesenchymal stem cells (MSCs) and bovine colostrum are potential therapies for the treatment of various degenerative and immune diseases. AIM: This study aimed to analyze the effect of MSCs on levels of tumor necrosis factor-Α (TNF-α) and macrophages M2 in the liver fibrosis of Wistar rats after 50% resection. METHODS: This study is a quasi-experimental post-test-only control group design to analyze the effect of giving bovine colostrum and MSCs to test animals on the process of regeneration of the remaining 50% liver with fibrosis. The study was conducted at the Stem Cell and Cancer Research Universitas Sultan Agung. The number of samples used was 40 male Wistar rats. The independent variables included MSC 1.000.0000 cells and bovine colostrum at a dose of 15 µL/g. Dependent variables used were macrophages M2 and levels of TNF-α ELISA. RESULTS: TNF-α levels on day 3 were (p = 0.001), day 7 were (p = 0.01), and day 10 were (p = 0.01) in liver tissue in various study groups analyzed using ELISA on day three*. The results showed differences which were significant between the control and treatment groups (p < 0.05). The expression of CD163 marked brown in liver tissue had more expression than the control group. CONCLUSION: The combination of MSCs and bovine colostrum can reduce TNF-α levels and significantly increase macrophages expression in the liver fibrosis of Wistar rats after 50% resection on the 3<sup>rd</sup>, 7<sup>th</sup>, and 10<sup>th</sup> days.
Liver regeneration in acute severe liver impairment: A clinicopathological correlation study
Katoonizadeh A., Nevens F., Pirenne J., Roskams T., Verslype C.
Failure of fibrotic liver regeneration in mice is linked to a severe fibrogenic response driven by hepatic progenitor cell activation
Burkly L., Csizmadia E., Hanto D.W., Kuramitsu K., Liu S.B., Otterbein L.E., Popov Y., Schuppan D., Sverdlov D.Y., Burkly L., Csizmadia E., Hanto D.W., Kuramitsu K., Liu S.B., Otterbein L.E., Popov Y., Schuppan D., Sverdlov D.Y.
Transient expression of cyclin D1 is sufficient to promote hepatocyte replication and liver growth in vivo
Albrecht J.H., Nelsen C.J., Rickheim D.G., Stanley M.W., Timchenko N.A.
Liver regeneration, growth factors, and amphiregulin
Khan Z., Michalopoulos G.K.
Three-dimensional bioprinted hepatorganoids prolong survival of mice with liver failure
Chi T., Du S., Hu D., Hu D., Huang P., Huang P., Lu X., Mao S., Mao Y., Pang Y., Peng W., Sang X., Sun L., Sun W., Sun W., Wang X., Wang X., Wang X., Wang Y., Xu H., Xu Y., Yang H., Zhang H., Zhao H., Zheng Y.-C., Zhong S.
Key aspects of the mesenchymal stem cells (MSCs) in tissue engineering for in vitro skeletal muscle regeneration
Chaudhuri B, Pramanik K.
Macrophages during the fibrotic process: M2 as friend and foe
Agudelo J.S.H., Braga T.T., Camara N.O.S., Camara N.O.S., Agudelo J.S.H., Braga T.T., Camara N.O.S., Camara N.O.S.
Transplantation of human bone marrow mesenchymal stromal cells reduces liver fibrosis more effectively than Wharton's jelly mesenchymal stromal cells
Balasubramanian S., Das A.K., Das A.K., Fakharuzi N.A., Fakiruddin K.S., Gupta P.K., Majumdar A.S., Nian L.M., Rahman A.Z.A., Rengasamy M., Sasidharan G., Siddikuzzaman, Singh G., Singh G., Swamynathan P., Thej C., Thej C., Zakaria Z.
Hepatocyte transplantation and advancements in alternative cell sources for liver-based regenerative medicine
Dhawan A., Fitzpatrick E., Lee C.A., Sinha S.
Gene expression profiling and secretome analysis differentiate adult-derived human liver stem/progenitor cells and human hepatic stellate cells
Berardis S., El Taghdouini A., Evraerts J., Lombard C., Lozano J.J., Najimi M., Rosseels V., Sancho-Bru P., Sokal E., Van Grunsven L.
Castagna F., Chiarella E., Ceniti C., Britti D.
Dairy
