Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6
Darlan D.M., Nasution I.P.A., Putra A., Muhar A.M., Ekasaputra V.M., Paramita D.A.
Abstract
Aim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion by regulating IL-6 at days 6 and 12. Methods Twenty-four PAs rat model weighing 250 g to 300 g were randomly allocated into 4 groups: sham (Sh), control (C), H-MSCs treatment group at dose 1.5 x 10<sup>6</sup> (T1) and 3 x 10<sup>6</sup> (T2). To induce H-MSCs, all MSCs population were incubated under hypoxia state (5% O<inf>2</inf> ), 5% CO<inf>2</inf>, and 37<sup>o</sup> C for 24 hours. Expression level of IL-6 was performed using ELISA. Morphological appearance of adhesion was observed by visualizing the existence of adhesion formation in intestinal. Results In this study we found that there was a trend of decrease of IL-6 level on day 6 following MSCs treatments. Interestingly, there was a significant decrease of IL-6 level on day 12 in all treatment groups. Also, no adhesion occurred in T2 group. Conclusions H-MSCs prevent PA development by suppressing the prolonged release of IL-6 at proliferation phase.
Mesenchymal stem cell paracrine factors in vascular repair and regeneration
Agrawal DK., Pankajakshan D, Agrawal DK., Pankajakshan D
Coordination of immune-stroma crosstalk by IL-6 family cytokines
West N.R.
IL-6 stimulates intestinal epithelial proliferation and repair after injury
Kuhn K.A., Kuhn K.A., Liu T.-C., Manieri N.A., Stappenbeck T.S.
Prevention and treatment of peritoneal adhesions in patients affected by vascular diseases following surgery: A review of the literature
Amato B., Amato M., Aprea G., Giuliani A., Paccone M., Rocca A., Russo A., Salzano A., Surfaro G., Tafuri D.
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Ghahari S., Jafari-Sabet M., Saeedi A.-R., Shishegar A.
Hypoxia changes chemotaxis behaviour of mesenchymal stem cells via HIF-1α signalling
Hu R., Li Z., Wang Y., Xu R., Xu W.
Battling adhesions: from understanding to prevention
Capella-Monsonis H, Dimitrios Z., Kearns S, Kelly J, Zeugolis DI.
Mesothelial cells in tissue repair and fibrosis
Birnie K., Herrick S.E., Lansley S., Lim C.-B., Mutsaers S.E., Prele C.M., Birnie K., Herrick S.E., Lansley S., Lim C.-B., Lim C.-B., Mutsaers S.E., Mutsaers S.E., Prele C.M., Prele C.M.
Mesenchymal stem cells induce regulatory t-cell population in human sle
Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B., Alif I., Darlan D.M., Ikhsan R., Kustiyah A.R., Munir D., Prasetio A., Putra A., Suntoko B.
Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition
Kustiyah AR., Muhar AM, Putra A, Trisnadi S, Kustiyah AR., Muhar AM, Putra A, Trisnadi S, Kustiyah AR., Muhar AM, Putra A, Trisnadi S
Putra A., Alif I., Makarim F.R., Ekasaputra V.M., Mughni A., Prasetya A., Nugrahadi D.E., Putranto I., Riwanto I.
Open Access Macedonian Journal of Medical Sciences
Li Z., Pu Z., Zhong P., Nian H., Ma S., Li J.
Surgery United States
