The effectivity of bovine colostrum and mesenchymal stem cell (Msc) on the improvement of alkaline phosphatase (alp) and takeda g-protein coupled receptor-5 (tgr5) level in post-hepatectomy wistar rats
Putra A., Prabowo E., Budiono B.P., Hendrawijaya A.E., Riwanto I.
Abstract
Background: The factor affecting the liver’s healing process after resection in liver malignancy is liver fibrosis. Bovine Colostrum (BC), as one of the anti-fibrosis products of the liver, improves hepatic fibrosis and reduces hepatocyte damage caused by carbon tetrachloride (CCl4). Meanwhile, Mesenchymal Stem Cell (MSC) is a new therapeutic source after resection. MSC is able to differentiate into specific cells in the healing process. This study aims to determine the effects of BC, MSC, and a combination of both in terms of increasing Alkaline Phosphatase (ALP) and Takeda G-Protein Coupled Receptor-5 (TGR5) levels in Wistar rats post hepatectomy 50% with liver fibrosis. Methods: This study is an experimental study with a randomized control trial design. Subjects were 25 Wistar rats (Rattus norvegicus) which were divided into 5 groups: Sham (surgery only), K (Control), K1 (CCL4 +BC), K2 (CCL4 + MSC), and K3 (CCL4 + MSC +BC). Blood was also taken to assess ALP and TGR5 at Day-3, Day-7, and Day-10 of treatment. Data were analyzed using SPSS version 25 for Windows. Results: A significant differences are found in ALP in the Sham group with K2 (p = 0.014) and the K2 group with K1 (p=0.026) on day 10. Significant differences in TGR5 are found in the Sham and K2 groups (p=0.009), control with K2 (p=0.014), K2 with K1 (p=0.007) on day 3. On day 7 in the control group with the combination (p=0.013). The test results showed that TGR5 on day 3 had a moderate significant strong correlation on ALP levels on day 10 (r=0.596; p=0.014). Conclusion: The combination of BC and MSC was not better than the administration of BC or MSC only in increasing ALP and TGR5 levels in rats with liver fibrosis after hepatectomy 50%. Based on this study, the administration of MSC is recommended.
Coenzyme Q concentration and total antioxidant capacity of human milk at different stages of lactation in mothers of preterm and full-term infants
Battino M., Bompadre S., Linde J., Maldonado J., Mataix J., Narbona E., Ochoa J.J., Quiles J.L., Ramirez-Tortosa M.C.
Anti-fibrotic effect of bovine colostrum in carbon tetrachloride-induced hepatic fibrosis
Gwak GY, Kwon YJ, Paik SW., Sinn DH, Gwak GY, Kwon YJ, Paik SW., Sinn DH, Gwak G-Y, Kwon YJ, Paik SW., Sinn DH
Liver fibrosis and hepatic stellate cells: Etiology, pathological hallmarks and therapeutic targets
He P., Lei J.-H., Wang C.-X., Yuan W.-G., Zhang C.-Y.
Role of phospholipase A2 in cholesterol gallstone formation is associated with biliary phospholipid species selection at the site of hepatic excretion: Indirect evidence
Asamoto Y., Hattori Y., Hyogo H., Kajihara T., Kajiyama G., Nakai K., Nishioka T., Ochi H., Sakomoto M., Sunami Y., Tazuma S., Tsuboi K., Yamashita G., Yasumiba S.
Serum alkaline phosphatase after extensive liver resection: A study in patients with biliary tract carcinoma
Hayakawa N., Kamiya J., Kanai M., Nagino M., Nimura Y., Uesaka K., Yamamoto H.
The mesenchymal stem cell secretome as an acellular regenerative therapy for liver disease
Driscoll J., Patel T.
Leptin is essential for the hepatic fibrogenic response to chronic liver injury
Farrell G.C., Leclercq I.A., Robertson G.R., Schriemer R.
Protease inhibitors from marine actinobacteria as a potential source for antimalarial compound
Bhaskara Rao K.V., Bhattacharyya A., Karthik L., Keswani T., Kumar G., Sarath Chandar S.
Bovine colostrum emerges as immune system modulator
Rona ZP.
Growth factors and antimicrobial factors of bovine colostrum
Aalto J., Pakkanen R., Pakkanen R.
Pranawa, Tjempakasari A., Hertanto D.M., Pramudya D., Pitaloka A.T.
Bali Medical Journal
Castagna F., Chiarella E., Ceniti C., Britti D.
Dairy
