The role of mesenchymal stem cells in decreasing interleukin-12 human systemic lupus erythematosus
Darlan D.M., Lubis R.R., Munir D., Putra A., Alif I.
Abstract
BACKGROUND: Systemic lupus erythematosus (SLE) disease is characterized by a loss of self-tolerance leading to a local tissue inflammation up to a massive systemic organ-spesific inflammation. Mesenchymal stem cells (MSCs) present immunomodulatory properties to control the over-activating immune responses in SLE through several mechanisms. However, the capability of MSCs to decrease interleukin (IL)-12 production in in vitro remains unclear. AIM: The aim of this study was to investigate the role of MSCs in decreasing the level of IL-12 derived from peripheral blood mononuclear cells (PBMCs) of SLE patients. METHODS: This study used a post-test control group design using a coculture of PBMCs from SLE and healthy patients with MSCs as the subjects. This study included five groups: sham (Sh), control (C), and treatment groups (T) treated by a co-culture MSCs with PBMCs at ratio dose of 1:1 (T1), 1:25 (T2), and 1:50 (T3), respectively, for 72 hours of incubation. The IL-12 levels was analysed by cytometric bead array (CBA) of flow cytometry. RESULTS: This study showed a significant decrease of IL-12 levels (p < 0.05) in T1 and T2 after 72 hours incubation of co-culture MSCs with PBMCs from SLE patient. CONCLUSION: MSCs could decrease the level of IL-12 in PBMCs of human SLE to control the inflammation of SLE disease.
'Yin-Yang' functions of transforming growth factor-β and T regulatory cells in immune regulation
Flavell R.A., Flavell R.A., Wan Y.Y., Flavell R.A., Flavell R.A., Wan Y.Y., Flavell R.A., Flavell R.A., Wan Y.Y.
TGF-β1 maintains suppressor function and Foxp3 expression in CD4 +CD25+ regulatory T cells
Gavin M., Letterio J.J., Marie J.C., Rudensky A.Y., Gavin M., Letterio J.J., Marie J.C., Rudensky A.Y.
In vitro regulation of il-6 and tgf-ß by mesenchymal stem cells in systemic lupus erythematosus patients
Alif I., Darlan D.M., Ikhsan R., Jusuf N.K., Munir D., Putra A., Alif I., Darlan D.M., Ikhsan R., Jusuf N.K., Munir D., Putra A., Alif I., Darlan D.M., Ikhsan R., Jusuf N.K., Munir D., Putra A., Alif I., Darlan D.M., Ikhsan R., Jusuf N.K., Munir D., Putra A., Alif I., Darlan D.M., Ikhsan R., Jusuf N.K., Munir D., Putra A., Alif I., Darlan D.M., Ikhsan R., Jusuf N.K., Munir D., Putra A.
IL-12 inhibits the TGF-β-dependent T cell developmental programs and skews the TGF-β-induced differentiation into a Th1-like direction
Holan V., Holan V., Pokorna K., Pokorna K., Prochazkova J., Prochazkova J.
A distal enhancer in II12b is the target of transcriptional repression by the STAT3 pathway and requires the basic leucine zipper (B-ZIP) protein NFIL3
Balouzian L., Johnson P.F., Murray P.J., O'Brien K., Qualls J.E., Schultz-Cherry S., Smale S.T., Smith A.M.
Interleukin-10 (IL-10) mediated suppression of IL-12 production in RAW 264.7 cells involves c-rel transcription factor
Boddupalli C.S., Hasnain S.E., Khan N., Mukhopadhyay S., Mukhopadhyay S., Rahim S.S.
Regulation of IL-10 and IL-12 production and function in macrophages and dendritic cells
Ban Y., Du Q., Li N., Ma X., Ma X., Wei F., Yan W., Zhang L., Zheng H.
Hypoxia-mesenchymal stem cells inhibit intra-peritoneal adhesions formation by upregulation of the il-10 expression
Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M., Muhar A.M., Munir D., Putra A., Warli S.M.
Positive regulatory role of IL-12 in macrophages and modulation by IFN-γ
Belladonna M.L., Bianchi R., Fallarino F., Fioretti M.C., Grohmann U., Orabona C., Puccetti P., Vacca C.
Clinical associations of IL-10 and IL-37 in systemic lupus erythematosus
Godsell J., Harris J., Hoi A., Kandane-Rathnayake R., Morand E.F., Nold M.F., Nold M.F., Rudloff I., Rudloff I.
Ichwan M., Farhat F., Ilyas S., Sembiring R.J., Munir D., Madiadipoera T., Putra A., Restimulia L., Amalina N.D., Alif I.
Medical Archives
Darlan D.M., Munir D., Jusuf N.K., Putra I.B., Putra A., Amalina N.D., Alif I.
Medicinski Glasnik
Wisnu Prajoko Y., Putra A., Prasetio A., Dina Amalina N.
Medicinski Glasnik
Putra A., Sarosa H., Trisnadi S., Amalina N.D., Mulyani S.P., Ibrahim S., Dewi A.M., Chodijah C.
Biosaintifika
