Effect of Combination of Probiotics, Ipomoea Batatas, and Zinc on Thickness of Instestinal Epithelium in Mice Model of Acetic Acid-Induced Colitis
Hussaana A., Yuniarifa C., Masfiyah M., Riza M., Tsuraya G.
Abstract
Background: The use of probiotics to treat colitis is unsatisfactory, leading to a need for the combination with purple sweet potatoes (Ipomoea Batatas) and zinc. Objectives: This study aims to determine the effect of the combination of probiotics, purple sweet potatoes, and zinc on epithelial thickness. Methods: This was a post-test-only randomized controlled group experimental study using 30 mice divided into six groups, including KN, K-, K+, P1, P2, and P3. All groups except KN were induced with 4% acetic acid on day 1, then each received treatment for five days. KN, K+, P1, and P2 groups were given distilled water, sulfasalazine 1.3 mg/20 g BW, probiotics 0.078 mg/20 gBW + purple sweet potatoes 0.00169 g/20 gBW, and probiotics 0.078 mg/20 gBW + zinc 0.052 mg/20 gBW, respectively. Meanwhile, P3 received a combination of probiotics 0.078 mg/20 gBW + purple sweet potatoes 0.00169 g/20 gBW + zinc 0.0052 mg/20 gBW. Histological preparations were stained with Hematoxylin-Eosin and examined under 400× magnification across 10 fields of view. Results: The average epithelial thicknesses were KN (75.48±5.09), K− (28.02±5.60), K+ (69.1±13.82), P1 (47.05±9.41), P2 (48.6±9.73), and P3 (71.57±14.31). One-way analysis of variance (ANOVA) showed a significant difference among the groups with a p-value <0.001. Post Hoc LSD test identified significant differences (p-value<0.05) among all group pairs except between K+ and P3 (p-value=0.134) as well as P1 and P2 (p-value=0.349), but P3 had the highest average thickness value (71.57±14.31). Conclusions: The combination of probiotics, purple sweet potatoes, and zinc had an effect on intestinal epithelial thickness in male BALB/c mice with ulcerative colitis.
Potensi Aktivitas Antioksidan Ubi Jalar (Ipomoea Batatas L.) Ungu dan Ekstrak Bunga Telang (Clitoria Ternatea L.) dalam Pembuatan Permen Jeli
Reni S.Y., Sinung P. F., Titha S. D.
Analisi Kandungan Zat Gizi pada Tepung Ubi Ungu (Ipomea Batatas Var Ayumurasaki) dengan Pengeringan Sinar Matahari dan Oven
Natsir N.A., Rijal M., Sere I.
Bakri A., Husin S., Salwan H.
Usefulness of sulfasalazine for patients with refractory-ulcerative colits
Sono M., Yazumi S., Yoshino T.
Bramantya N.A, Setiawan B.
Switching between three types of mesalazine formulation and sulfasalazine in patients with active ulcerative colitis who have already received high-dose treatment with these agents
Harada K., Hirai M., Hiraoka S., Inokuchi T., Kato J., Kinugasa H., Oka S., Okada H., Takahara M., Yamamoto S., Yamasaki Y., Yasutomi E.
Pribaningrum A.
Budi H.C., Intan N.T.A
Wahyu P. P.
Abdurrachman L. H.
