The effect of combined doxorubicin and Dioscorea esculenta (Lour.) Burk. on apoptosis induction in human breast cancer cell
Wahyuningsih M.S.H., Mubarika S., Fatmawati D.
Abstract
Chemotherapy for breast cancer is largely ineffective, but innovative combinations of chemotherapeutic agents and natural compounds represent a promising strategy. In our present study, the combination of Doxorubicin (Dox) and ethanol extract of Dioscorea esculenta (Lour.) Burk. tuber was found to have a synergistic effect on T47D human breast cancer cell line. In this study, we investigated the apoptotic effect of the combination on T47D human breast cancer cells and normal fibroblasts cell line and its effects on the expression of Caspase-3 and cleaved poly (ADP-Ribose) Polymerase-1 (cPARP-1) protein. Methods: T47D cell lines and fibroblasts cells were treated with the combination of Dox and ethanol extract of Dioscorea esculenta (Lour.) Burk. Apoptotic effect of the combination was determined using flow cytometry assay. Protein expressions were determined by immunocytochemistry staining. Results: The percentage of apoptotic cells were significantly higher in T47D cell lines (77.78 %) than that of in fibroblast cells (23 %) (p < 0.05). The expression of Caspase 3 (84.53 %) and cPARP-1 (83.36 %) were significantly higher in the cancer cell lines than those of normal cells (p < 0.05). Conclusion: These results indicate that the combination of doxorubicin and Dioscorea esculenta (Lour.) Burk. is a promising candidate for the treatment of breast cancer cells.
Diosgenin, a plant-derived sapogenin, exhibits antiviral activity in vitro against hepatitis C virus
Chang T.-Y., Hsieh T.-C., Hsu J.T.-A., Hsu J.T.-A., Lin W.-H., Pan K.-L., Pan K.-L., Wang Y.-J.
Antioxidants in cancer therapy; their actions and interactions with oncologic therapies
Brignall M.S., Brignall M.S., Lamson D.W., Lamson D.W., Lamson D.W.
Caspase family proteases and apoptosis
Cong R.-S., Fan T.-J., Han L.-H., Liang J., Cong R.-S., Fan T.-J., Han L.-H., Liang J.
No Title
Arifin M.Z., Bisri T., Faried A., Shahib M.N., Wiriadisastra K.
Diosgenin, a naturally occurring steroid, suppresses fatty acid synthase expression in HER2-overexpressing breast cancer cells through modulating Akt, mTOR and JNK phosphorylation
Chiang C.-T., Lin J.-K., Lin J.-K., Tsai S.-J., Way T.-D.
Saponins as cytotoxic agents: A review
Galanty A., Podolak I., Sobolewska D.
Anticancer effect and cell cycle analysis of ethanolic extract of gembili (D. Esculanta L burkill) on T47D breast cancer cell line
Soetoko D.F., Sumarno
In vitro antioxidant studies of Dioscorea esculenta (Lour). Burkill
Mohan V.R., Murugan M.
A survey on phytochemical and bioactivity of plant extracts from Malaysian forest reserves
Ahmad I.B., Din L.B., Farina A.H., Ling C.Y., Mohd Razehar A.R., Nardiah Rizwana J., Nazlina I., Shariffah Muzaimah S.A., Siti Noraziah A.Z., Yaacob W.A.
Anti-inflammatory studies of yam (Dioscorea esculenta) extract on wistar rats
Ajaiyeoba E.O., Olayemi J.O.
