Secretome of Hypoxia-Preconditioned Mesenchymal Stem Cells Enhance Angiogenesis in Diabetic Rats with Peripheral Artery Disease
Ilyas S., Sembiring R.J., Lindarto D., Putra A., Hasan R., Syafril S., Sazli B.I., Pranoto A.
Abstract
Background: Lower limb peripheral artery disease (PAD) is the main risk of diabetes melli-tus which result to high mortality rate. Approximately, 50% of patients who receive several treatments have passed away or lost limbs at a year’s follow-up. Secretome of hypoxia mesenchymal stem cells (S-MSCs) contains several active soluble molecules from hypox-ia MSCs (H-MSCs) that capable inducing anti-inflammatory and vascular regeneration in PAD. Objective: In this study, we investigated the therapeutic potential of S-MSCs in improving dynamic function and angiogenesis of PAD diabetic rats. Methods: The PAD was established by the incision from the groin to the inner thigh and distal ligation of femoral arteries in rats with diabetes. Rats were administered with 200 µL and 400 µL S-MSCs that successfully filtrated using tangential flow filtration (TFF) system based on various molecular weight cut-off categories intravenously. ELISA assay was used to analyze the cytokines and growth factors contained in S-MSCs. Tarlov score were examined at day 1, 3, 5, 7, 10 and 14. The rats were sacrificed at day 14 and muscle tissues were collected for immunohistochemistry (IHC) and gene expression analysis. Results: ELISA assay showed that S-MSCs provides abundant level of VEGF, PDGF, bFGF, IL-10 and TGFβ. In vivo adminis-tration of S-MSCs remarkably enhanced the Tarlov score. S-MSCs improved angiogenesis through enhancing VEGF gene expression and significantly increasing CD31 positive area in muscle tissue of PAD diabetic rats. Conclusion: Our findings suggest that S-MSCs could improves dynamic function and angiogenesis in PAD diabetic rats.
Development of secretome-based strategies to improve cell culture protocols in tissue engineering
Alaminos M., Alaminos M., Blanco-Elices C., Blanco-Elices C., Blanco-Elices C., Campos F., Campos F., Cases-Perera O., Cases-Perera O., Chato-Astrain J., Chato-Astrain J., Crespo P.V., Crespo P.V., Garzon I., Garzon I., Martin-Piedra M.A., Martin-Piedra M.A., Miranda-Fernandez C., Sanchez-Montesinos I., Sanchez-Montesinos I.
Endothelial dysfunction and diabetes: Effects on angiogenesis, vascular remodeling, and wound healing
Bir S.C., Kevil C.G., Kolluru G.K.
The Role of Hypoxic Mesenchymal Stem Cells Con-ditioned Medium in Increasing Vascular Endothelial Growth Factors (VEGF) Levels and Collagen Synthesis to Accelerate Wound Healing
Chodidjah C., Putra A, Sa'dyah NAC, Sunarto H, Tjipta A, Trisnadi S, Cho-didjah C., Putra A, Sa'dyah NAC, Sunarto H, Tjipta A, Trisnadi S, Chodidjah C., Putra A, Sa'dyah NAC, Sunarto H, Tjipta A, Trisnadi S, Chodidjah C, Putra A, Sa'dyah NA, Sunarto H, Tjipta A, Trisnadi S, Chodidjah C., Putra A, Sa'dyah NAC, Sunarto H, Tjipta A, Trisnadi S, Chodidjah C, Putra A, Sa'dyah NAC, Sunarto H, Tjipta A, Trisnadi S, Chodidjah C., Putra A, Sa'dyah NAC, Sunarto H, Tjipta A, Trisnadi S
Efficacy of topical and systemic transplantation of mesenchymal stem cells in a rat model of diabetic ischemic wounds
Cao Y., Cao Y., Cao Y., Chen X., Chen X., Chen X., Chen X., El Akkawi M.M., El Akkawi M.M., El Akkawi M.M., Li C., Li K., Li K., Li K., Liang J., Liang J., Liang J., Liao X., Liao X., Liao X., Liu H., Liu H., Liu H., Xie G., Xie G., Xie G., Yan J., Yan J., Yan J.
Comparative analysis of mouse bone marrow and adipose tissue mesenchymal stem cells for critical limb ischemia cell therapy
Asadi-Yousefabad S.-L., Daneshi S., Nammian P., Razban V., Razban V., Sheikhha M.H., Tabei S.M.B., Tabei S.M.B.
Adenoviral transfer of HIF-1α enhances vascular responses to critical limb ischemia in diabetic mice
Bosch-Marce M., Fox-Talbot K., Sarkar K., Semenza G.L., Steenbergen C.
Angiogenesis in diabetic mouse model with critical limb ischemia; cell and gene therapy
Asadi-Yousefabad S.-L., Daneshi S., Fallahi J., Nammian P., Razban V., Razban V., Sheikhha M.H., Tabei S.M.B., Tabei S.M.B.
Enrichment of vascular endothelial growth factor secreting mesenchymal stromal cells enhances therapeutic angiogenesis in a mouse model of hind limb ischemia
BAE S.E.O.N.G.-H.O., CHOI D., CHOI D., JUNG S., LEE M., PARK J.S.
Pretreatment of Diabetic Adipose-derived Stem Cells with mitoTEMPO Reverses their Defective Proangiogenic Function in Diabetic Mice with Critical Limb Ischemia
Chen G., Chen Y., Gao H., Li C., Li C., Lian K., Wang Q., Yang M., Zhao S.
Cellular death, reactive oxygen species (ROS) and diabetic complications review-Article
Dos Anjos P.M.F., Nogueira-Machado J.A., Villar-Delfino P.H., Volpe C.M.O.
Guan J., Niu H., Dang Y., Guan Y., Wen J., Zhai J.
Journal of Controlled Release
Darlan D.M., Mangunatmadja I., Putra A., Japardi I., Rusda M., Daulay R.S., Sofyani S., Silvana S., Andreas Y.
Baghdad Science Journal
Journal of Extracellular Vesicles
Ahdut-HaCohen R., Sionov R.V.
Biomedicines
Sumarawati T., Putra A., Jessika C.
Biosaintifika
Putra A., Mulyani S.P., Rahardja C.K.
Biosaintifika
Sadyah N.A.C., Putra A., Heri-Nugroho, Riwanto I.
International Journal of Biomedicine
